Sattya Arimurti

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Yeya Nepsan

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Vivien Ajeng Pradita

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Nuruli Fifi Anggraeni

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Sutoyo Sutoyo

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Siswanto Siswanto

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Tantri Raras Ayuningtyas

Ijen Geopark, Bondowoso

Esti Utarti

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

DOI: https://doi.org/10.19184/lsb.v1i2.44097

ABSTRACT 

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code Isolate from Hutan Pelangi (IHP). These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). The hemolysis reactions offer simple tests to ease the analysis of pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest based on their hemolysis reactions. Pathogenicity tests are performed through hemolysis reactions. The hemolysis test is performed by inoculating bacterial isolates on blood agar media after incubating for 48 hours. The results showed that as many as 123 (80.39%) bacterial isolates had type ß hemolysis thus being pathogenic, meanwhile as many as 30 (19.61%) bacterial isolates had type γ hemolysis so they were non-pathogenic.

Keywords: bacteria, hemolysis, Ijen geopark.