Putry Lazimatu Ziyaroh

Biology Department, Faculty of Mathematics and Natural Sciences, Jember University, East Java, Indonesia

Waki’atil Rosida

Biology Department, Faculty of Mathematics and Natural Sciences, Jember University, East Java, Indonesia

Edia Fitri Dwinianti

Biology Department, Faculty of Mathematics and Natural Sciences, Jember University, East Java, Indonesia

Mukhamad Su’udi

Biology Department, Faculty of Mathematics and Natural Sciences, Jember University, East Java, Indonesia

DOI: https://doi.org/10.19184/lsb.v2i2.53562

ABSTRACT 

Dendrobium crumenatum Sw. is one of the epiphytic orchids with high ecological and economic value. This study aims to examine the effect of media containing organic compounds enriched with fertilizers for in vitro propagation of D. crumenatum seeds. This culture medium contains organic materials such as potatoes, bananas, coconut water, charcoal, sucrose, and foliar fertilizer. This medium was designed to provide macro, micro, and growth hormone nutrients that support seed growth. The research procedure included sterilization of orchid seeds, media preparation, seed sowing, and growth observation for about two months. The results showed that orchid seeds experienced optimal growth in media with the addition of coconut water and other organic compounds. The formulation of organic-based media with the addition of coconut water and other supporting compounds is effective in supporting the in vitro growth of orchids, providing opportunities for efficient propagation of orchid species. Explant growth reached 1-2 cm at the 68th day after sowing, with a fresh green colour was a indicator of normal growth. The even distribution of shoots indicated that the media provideed sufficient nutrients and space competition between explants. In sum, our simple tissue culture media produced from a mixture of organic materials proved reliable function and suited for growing D. crumenatum orchid seeds to produce plantlets, and to a further extent it would support conservation efforts.

Keywords: Conservation, organic materials, pigeon orchid, simple media, tissue culture.