Rian Oktiansyah

Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Mardiyah Pratiwi

Rumah Sakit Khusus Gigi dan Mulut, South Sumatra, Indonesia

Sully Pudja Kharisma

Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Ahmad Rizki Fauzan

Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Riza Utami

Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Dea Amelia

Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Noviyanto

Technician of biology laboratory, Integrated Laboratory, Universitas Islam Negeri Raden Fatah, Palembang, Indonesia

Graduate School of Sciences, Faculty of Mathematic and Natural Sciences, University of Sriwijaya, Jl. Padang SelasaaNo. 524, Palembang 30129, South Sumatra, Indonesia

Sakinah Salman Ahmad Nasution

Graduate School of Sciences, Faculty of Mathematic and Natural Sciences, University of Sriwijaya, Jl. Padang SelasaaNo. 524, Palembang 30129, South Sumatra, Indonesia

DOI: https://doi.org/10.19184/lsb.v2i2.52579

ABSTRACT 

The study investigates the bioactivity of endophytic fungi isolated from Eleocharis dulcis (Burm.f.) Trin. ex Hensch., focusing on their potential as alternative sources of bioactive compounds. E. dulcis, a plant commonly found in wetland ecosystems, has traditionally been used for its medicinal properties. However, the massive extraction of the plant may lead to ecological imbalance, prompting the exploration of endophytic fungi as a sustainable alternative. The objective of this research is to isolate, identify, and evaluate the antimicrobial and antioxidant properties of endophytic fungi from Eleocharis dulcis. Endophytic fungi were isolated aseptically, followed by morphological identification. Antioxidant and antimicrobial assays were conducted to assess the bioactivity of the fungal extracts. Antioxidant activity was evaluated using the DPPH method, while antibacterial activity was assessed using the disc paper diffusion method. The results revealed that several isolates (from leaves: LP1 – LP6, from root: RP1 – RP3) exhibited significant antimicrobial activity against Escherichia coli and Staphylococcus aureus, with Penicillium spp. and Cladosporium spp. showing the highest levels of bioactivity. Additionally, all fungal isolates demonstrated very strong antioxidant activity, with most achieving an IC50 below 20 μg/mL. In conclusion, the findings suggest that endophytic fungi from E. dulcis represent a valuable source of bioactive compounds with potential applications in pharmaceuticals. Furtheryresearch is required to isolate and characterizeethe active compounds, as well as to evaluate their efficacy through in vivo studies.

Keywords: Antibacterial, Antioxidant, Eleocharis dulcis, Endophytic fungi.