Sindhi Wahidatul Ikrimah

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Lailiyah Maulidatul Hasanah

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Tri Ratnasari

Faculty of Agriculture, University of Jember, Indonesia

Nurfajri Eka Fitri

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

Mukhamad Su'udi

Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Indonesia

DOI: https://doi.org/10.19184/lsb.v2i1.48632

ABSTRACT 

Dendrobium crumenatum, known as the pigeon orchid, is a type of orchid that is used as medicine by local people. Morphological identification to the D. crumenatum has an obstacle due to the similar reproductive stucture to the same genus of D. heterocarpum. DNA barcoding is an alternative technique for identifying D. crumenatum which provides a more objective measure based on genetic data. One of the molecular markers that is reliable and widely used in DNA barcoding is ITS2. The purpose of this research is to identify the ITS2 sequence as a molecular barcode effective for D.crumenatum. Genomic DNA isolation of Dendrobium crumenatum was carried out with the CTAB method (Cetyl Trimethyl Ammonium Bromide) and DNA amplification using PCR. The results showed that DNA barcoding research using the ITS2 sequence in D. crumenatum provides specific results to differentiate D. crumenatum from other Dendrobium species. The similarity of flower morphology in D. crumenatum and D. formosum proposed as the cause of close proximity shown in phylogenetic tree. Based on these results, it is highly recommended to use the ITS2 sequence as a molecular marker for barcoding orchids, especially D. crumenatum since its capability for differentiating between species with morphological similarities.

Keywords: Dendrobium crumenatum, DNA Barcoding, ITS2.