Nurul Insani Shullia

Department Biology Education, Faculty of Teacher Training and Education, Jember University, City Jember, Indonesia

Kuswati

Department Biology Education, Faculty of Teacher Training and Education, Jember University, City Jember, Indonesia

Aditya Kurniawan

Department Biology Education, Faculty of Teacher Training and Education, Jember University, City Jember, Indonesia

Hajar Syifa Fiarani

Department Biology Education, Faculty of Teacher Training and Education, Jember University, City Jember, Indonesia

DOI: https://doi.org/10.19184/lsb.v1i1.40052

ABSTRACT 

The yellow dwarf honey bee, Apis florea is well distributed in South Asia to South East Asia. This species is expanded and introduced area from their original distribution. However, the distribution of this honey bee in Indonesia is unexplored. The cytochrome c oxidase subunit 1 (COX1) gene is successful to detect the original geographic of introduced A. florea found in Egypt. The A. florea specific primer of COX1 gene is needed to produce the molecular marker for geographical origin detection. Thus, this study aims to in silico design the COX1 gene primer of A. florea using Primer3 and Primer-BLAST. This study results in the best candidate primer of Af6_COX1_F and Af6_COX1_R primers that start from 212 to 983 regarding the A. florea COX1 gene (NC_021401) and produce 772 bp of the amplicon. The melting temperature of the forward primer is 54. 63 and 55.58°C, GC content of 40 and 45%, GC clamp of 3 and 2 for forward and reverse primer, respectively. There are no secondary structures of those primers and 100 % homolog with A. florea COX1 sequences, thus those primer is the potential as the geographical origin marker of A. florea.

Keywords: DNA barcoding, maturase K, orchids.